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Abstract
Objectives: The aims of the present study were firstly, to evaluate the effect of estrogen on
the cytology of buccal mucosa during the menstrual cycle and at menopause, and, secondly,
to analyze the presence of estrogen receptors in the epithelial cells of buccal mucosa
using immunohistochemical methods. Methods: The cytological samples of buccal mucosa from 10 healthy young women (mean age 24
years) were taken on every day of the menstrual cycle and stained according to Papanicolaou.
A single cytological sample of buccal mucosa was also collected from 20 healthy postmenopausal
women (mean age 66 years) using no hormone replacement therapy. For each specimen,
a maturation index was calculated on the basis of percentages of the three (parabasal,
intermediate and superficial) cell types. Moreover, 50 incisional biopsies were taken
from buccal mucosa of 50 young healthy women (19 years old) for analysis of estrogen
receptor expression by immunohistochemistry using an indirect immunoperoxidase technique.
Results: On cytohormonal evaluation, intermediate cells were dominant in postmenopausal women
and also in young women at all stages of the menstrual cycle indicating a nearly complete
cell maturation. Estrogen receptor positive cells were not detected in buccal epithelium
by immunohistochemistry. Conclusions: As the cell pattern of buccal mucosa indicated a nearly complete cell maturation
both in young and in postmenopausal women, estrogen may not be the only factor causing
maturational changes in buccal epithelial cells. Alternatively, the antibody used
in immunohistochemistry may not detect the epitope of the estrogen receptor present
in buccal mucosa or the level of expression of this protein may be under the detection
limit.
Keywords
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Article info
Publication history
Accepted:
January 23,
1997
Received in revised form:
January 16,
1997
Received:
December 2,
1996
Identification
Copyright
© 1997 Published by Elsevier Inc.
