Highlights
- •A novel approach combining epidemiological and in-vitro analyses is used to study the influence of C-reactive protein on cardiovascular health in elderly women.
- •The serum level of C-reactive protein is associated with blood pressure in elderly women.
- •C-reactive protein in elderly women limits the proliferative capacity of endothelial cells.
- •C-reactive protein in elderly women reduces the angiogenic potential of endothelial cells.
Abstract
It is hypothesized that chronic systemic inflammation contributes to the age-related
decline in cardiovascular function. The aim of the present study was to combine an
assessment of the relationship between the serum level of C-reactive protein (CRP)
and systolic and diastolic blood pressure in 108 elderly women (65 and 70 years) with
an in-vitro exploration of the effects of CRP on the proliferative and angiogenic potential of
endothelial cells exposed to serum in elderly women. Based on the median CRP level
in our population, LowCRP (CRP < 1.3 mg/L) and HighCRP (>1.3 mg/L) groups were identified. Body mass index, waist circumference, systolic blood
pressure (SBP) and diastolic blood pressure (DBP) were significantly higher in the
HighCRP group than in the LowCRP group (p < 0.05). The influence of CRP on SBP and DBP remained significant after adjustments
for BMI and use of antihypertensive medication (p < 0.05). When adjusting for waist circumference the observed influence of CRP on SPB
was attenuated (p = 0.062). We next evaluated the ability to form capillary tubes (angiogenesis assay)
and the proliferation rate of endothelial cells exposed to the sera of elderly women.
Increased serum CRP levels were associated with an increased doubling time of endothelial
cells (R2 = 0.39; p < 0.05) and decreased capillary tube length (R2 = 0.30; p < 0.05), indicating a reduction in the proliferation rate of endothelial cells and angiogenic
potential. In conclusion, chronic inflammation influences blood pressure in elderly
women and compromises endothelial cell function, thus contributing to the age-related
decline in vascular health.
Keywords
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Article info
Publication history
Published online: April 23, 2016
Accepted:
April 18,
2016
Received in revised form:
April 9,
2016
Received:
November 4,
2015
Identification
Copyright
© 2016 Elsevier Ireland Ltd. All rights reserved.
