Abstract
Objectives
The menopause is associated with an increase of inflammatory markers (C-reactive protein,
fibrinogen), cytokines (INFγ, TNF, etc.) and blood lipoproteins. In vitro, CRP, LDL
and fibrinogen can modulate or potentiate interleukines production by monocytes. The
aim of this work was to study, the relationships in vivo between hs-CRP, fibrinogen,
lipoproteins and the phenotype of circulating monocytes.
Methods
The monocytes phenotype, in postmenopausal women (n = 26) without history of cardiovascular disease, was determined, by flow cytometry,
measuring granularity and CD14, HLA-DR and CD62-L antigens expression. Blood monocytes
were divided in CD14+dim monocytes (low CD14 expression) and CD14+bright monocytes
(high CD14 expression).
Results
HLA-DR was negatively correlated with hs-CRP and fibrinogen. The relationships between
ApoB, LDL/ApoB ratio and CD14 expression was restricted to the CD14+bright monocytes.
Blood lipids, i.e. total cholesterol, LDL-c and ApoB were correlated with the granularity
of both subsets. CD14+dim monocytes were characterized by a low granularity and CD62-L
expression.
Conclusions
Our data show that fibrinogen and hs-CRP are correlated with a reduced antigen-presenting
capacity. Expression of CD14 on CD14+bright monocytes is negatively associated to
atherogenic LDL. Blood monocytes granularity was positively correlated with serum
lipids indicating that monocytes could uptake modified LDL in circulation and not
restricted to subendothelial space.
Keywords
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Article info
Publication history
Accepted:
December 13,
2005
Received in revised form:
December 9,
2005
Received:
July 11,
2005
Identification
Copyright
© 2006 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
