Abstract
Background
Hormone replacement therapy (HRT) has been used in treatment of various menopausal
disorders. It has been well documented that HRT increases the amount of dermal collagen
and skin thickness in vivo. However little is known about the effects of female sex
hormones on dermal fibroblasts in vitro.
Objective
The aim of this study is to determine whether or not 17β-estradiol affects mRNA expression
and production of type I collagen, matrix metalloproteinases-1 (MMP-1), tissue inhibitor
metalloproteinases-1 (TIMP-1) or transforming growth factor-β1 (TGF-β1) by human dermal
fibroblasts.
Methods
Fibroblasts were cultured with and without 17β-estradiol for 6 h. We evaluated the changes of mRNA expressions and protein production of type I collagen,
MMP-1, TIMP-1 and TGF-β1.
Results
The mRNA expressions of collagen α1(I), MMP-1, TIMP-1, TGF-β1 were not changed by
17β-estradiol stimulations at a concentration of 10−12 to 10−8 M. However, 17β-estradiol at concentrations of 10−12 and 10−10 M exhibited inhibitory effects on proMMP-1, but not type I collagen or TIMP-1 synthesis.
The synthesis of TGF-β1 by fibroblasts stimulated with 10−8 M of estradiol was significantly increased as compared with the control. However,
the level of TGF-β type II receptor phosphorylation was not elevated under the same
conditions.
Conclusion
Suppressed synthesis of MMP-1 at a low concentration of 17β-estradiol may be partly
involved in the dermal tissue remodeling to inhibit the degradative change.
Keywords
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Article info
Publication history
Accepted:
August 23,
2005
Received in revised form:
August 9,
2005
Received:
January 17,
2005
Identification
Copyright
© 2005 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
