Maturitas
Volume 55, Issue 1 , Pages 76-81 , 20 August 2006

Effects of a red clover extract (MF11RCE) on endometrium and sex hormones in postmenopausal women

  • Martin Imhof

      Affiliations

    • Division of Obstetrics and Gynecology, Department of Gynecological Endocrinology and Reproductive Medicine, University of Vienna, Währinger Gürtel 18-20, A-1090 Vienna, Austria
    • Corresponding Author InformationCorresponding author. Tel.: +43 1 40400 2816; fax: +43 1 40400 2817.
  • ,
  • Anca Gocan

      Affiliations

    • Study Center Med XIX Grinzingerstr. 83, A-1190 Vienna, Austria
  • ,
  • Franz Reithmayr

      Affiliations

    • Study Center Med XIX Grinzingerstr. 83, A-1190 Vienna, Austria
  • ,
  • Markus Lipovac

      Affiliations

    • Division of Obstetrics and Gynecology, Department of Gynecological Endocrinology and Reproductive Medicine, University of Vienna, Währinger Gürtel 18-20, A-1090 Vienna, Austria
  • ,
  • Claudia Schimitzek

      Affiliations

    • Division of Obstetrics and Gynecology, Department of Gynecological Endocrinology and Reproductive Medicine, University of Vienna, Währinger Gürtel 18-20, A-1090 Vienna, Austria
  • ,
  • Peter Chedraui

      Affiliations

    • Institute of Biomedicine, Facultad de Ciencias Medicas, Universidad Católica de Santiago de Guayaquil, Av. Carlos Julio Arosemena km 1 1/2, Guayaquil, Ecuador
  • ,
  • Johannes Huber

      Affiliations

    • Division of Obstetrics and Gynecology, Department of Gynecological Endocrinology and Reproductive Medicine, University of Vienna, Währinger Gürtel 18-20, A-1090 Vienna, Austria

Received 4 November 2005 ,Revised 5 January 2006 ,Accepted 6 January 2006.

References 

  1. Barrett-Connor E, Grady D, Stefanick ML. The rise and fall of menopausal hormone therapy. Annu Rev Public Health. 2005;26:115–140
  2. North American Menopause Society. Treatment of menopause-associated vasomotor symptoms: position statement of The North American Menopause Society. Menopause. 2004;11:11–33
  3. Lock M. Menopause in cultural context. Exp Gerontol. 1994;29:307–317
  4. Atkinson C, Compston JE, Day NE, et al. The effects of phytoestrogen isoflavones on bone density in women: a double-blind, randomized, placebo-controlled trial. Am J Clin Nutr. 2004;79:326–333
  5. Adlercreutz H, Mazur W. Phytoestrogens and Western diseases. Ann Med. 1997;29:95–120
  6. Morito K, Aomori T, Hirose T, et al. Interaction of phytoestrogens with estrogen receptors alpha and beta (II). Biol Pharm Bull. 2002;25:48–52
  7. Setchell KD, Cassidy A. Dietary isoflavones: biological effects and relevance to human health. J Nutr. 1999;129:S758–S767
  8. Yoon SL, Horne CH. Herbal products and conventional medicines used by community-residing older women. J Adv Nurs. 2001;33:51–59
  9. Mahady GB, Parrot J, Lee C, et al. Botanical dietary supplement use in peri- and postmenopausal women. Menopause. 2003;10:65–72
  10. Krenn L, Unterrieder I, Ruprechter R. Quantification of isoflavones in red clover by high-performance liquid chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2002;777:123–128
  11. Hur H, Rafii F. Biotransformation of the isoflavonoids biochanin A, formononetin, and glycitein by Eubacterium limosum. FEMS Microbiol Lett. 2000;192:21–25
  12. Nachtigall LB, La Grega L, Lee WW, et al. The effects of isoflavones derived from red clover on vasomotor symptoms and endometrial thickness. In: 9th International Menopause Society World Congress on the Menopause, Yokohama. Japan, 17–21 October, Bologna, Monduzzi Editore. 1999;p. S331–S336
  13. Baber RJ, Templeman C, Morton T, et al. Randomized placebo-controlled trial of an isoflavone supplement and menopausal symptoms in women. Climacteric. 1999;2:85–92
  14. Clifton-Bligh PB, Baber RJ, Fulcher GR, et al. The effect of isoflavones extracted from red clover (Rimostil) on lipid and bone metabolism. Menopause. 2001;8:259–265
  15. Upmalis DH, Lobo R, Bradley L, et al. Vasomotor symptom relief by soy isoflavone extract tablets in postmenopausal women: a multicenter, double-blind, randomized, placebo-controlled study. Menopause. 2000;7:236–242
  16. Penotti M, Fabio E, Modena AB, et al. Effect of soy-derived isoflavones on hot flushes, endometrial thickness, and the pulsatility index of the uterine and cerebral arteries. Fertil Steril. 2003;79:1112–1117
  17. Nikander E, Rutanen EM, Nieminen P, et al. Lack of effect of isoflavonoids on the vagina and endometrium in postmenopausal women. Fertil Steril. 2005;83:137–142
  18. Sammartino A, Di Carlo C, Mandato VD, et al. Effects of genistein on the endometrium: ultrasonographic evaluation. Gynecol Endocrinol. 2003;17:45–49
  19. Duncan AM, Underhill KE, Xu X, et al. Modest hormonal effects of soy isoflavones in postmenopausal women. J Clin Endocrinol Metab. 1999;84:3479–3484
  20. Balk JL, Whiteside DA, Naus G, et al. A pilot study of the effects of phytoestrogen supplementation on postmenopausal endometrium. J Soc Gynecol Investig. 2002;9:238–242
  21. Hale GE, Hughes CL, Robboy SJ, et al. A double-blind randomized study on the effects of red clover isoflavones on the endometrium. Menopause. 2001;8:338–346
  22. Scambia G, Mango D, Signorile PG, et al. Clinical effects of a standardized soy extract in postmenopausal women: a pilot study. Menopause. 2000;7:105–111
  23. Unfer V, Casini ML, Costabile L, et al. Endometrial effects of long-term treatment with phytoestrogens: a randomized, double-blind, placebo-controlled study. Fertil Steril. 2004;82:145–148
  24. Munro IC, Harwood M, Hlywka JJ, et al. Soy isoflavones: a safety review. Nutr. Rev. 2003;61:1–33
  25. Peeters PH, Keinan-Boker L, van der Schouw YT, et al. Phytoestrogens and breast cancer risk: review of the epidemiological evidence. Breast Cancer Res Treat. 2003;77:171–183
  26. Piersen CE, Booth NL, Sun Y. Chemical and biological characterization and clinical evaluation of botanical dietary supplements: a phase I red clover extract as a model. Curr Med Chem. 2004;11:1361–1374
  27. Barnes S. Phyto-oestrogens and osteoporosis: what is a safe dose?. Br J Nutr. 2003;89:S101–S108
  28. Burd ID, Bachmann GA. Androgen replacement in menopause. Curr Womens Health Rep. 2001;1:202–205
  29. Soares CN, Poitras JR, Prouty J. Effect of reproductive hormones and selective estrogen receptor modulators on mood during menopause. Drugs Aging. 2003;20:85–100
  30. Davis S. Testosterone deficiency in women. J Reprod Med. 2001;46:S291–S296
  31. Rohr UD. The impact of testosterone imbalance on depression and women's health. Maturitas. 2002;41:S25–S46
  32. Moyer DL. Risk of depression increases during menopause. In: Proceedings of the APA 157th Annual Meeting (abstract NR768), Presented May 5. 2004;medscape.com
  33. Wu AH, Stanczyk FZ, Martinez C, et al. A controlled 2-mo dietary fat reduction and soy food supplementation study in postmenopausal women. Am J Clin Nutr. 2005;81:1133–1141
  34. Foth D, Nawroth F. Effect of soy supplementation on endogenous hormones in postmenopausal women. Gynecol Obstet Investig. 2003;55:135–138
  35. Atkinson C, Warren RM, Sala E, et al. Red-clover-derived isoflavones and mammographic breast density: a double-blind, randomized, placebo-controlled trial. Breast Cancer Res. 2004;6:R170–R179
  36. Knight DC, Howes JB, Eden JA. The effects of Promensil, an isoflavone extract, on menopausal symptoms. Climacteric. 1999;2:79–84
  37. Jeri AR. The use of an isoflavone supplement to relieve hot flushes. The Female Patient. 2002;27:35–37
  38. Pino AM, Valladares LE, Palma MA, et al. Dietary isoflavones affect sex hormone-binding globulin levels in postmenopausal women. J Clin Endocrinol Metab. 2000;85:2797–2800
  39. Murkies AL, Wilcox G, Davis SR. Clinical review 92: phytoestrogens. J Clin Endocrinol Metab. 1998;83:297–303
  40. Piersen CE. Phytoestrogens in botanical dietary supplements: implications for cancer. Integr Cancer Ther. 2003;2:120–138
  41. Setchell KD, Brown NM, Desai P, et al. Bioavailability of pure isoflavones in healthy humans and analysis of commercial soy isoflavone supplements. J Nutr. 2001;131:1362S–1375S
  42. Dornstauder E, Jisa E, Unterrieder I, et al. Estrogenic activity of two standardized red clover extracts (Menoflavon(R)) intended for large scale use in hormone replacement therapy. J Steroid Biochem Mol Biol. 2001;78:67–75

PII: S0378-5122(06)00010-7

doi: 10.1016/j.maturitas.2006.01.006

Maturitas
Volume 55, Issue 1 , Pages 76-81 , 20 August 2006